کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738718 | 1046751 | 2009 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CFTR mediates cadmium-induced apoptosis through modulation of ROS level in mouse proximal tubule cells
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کلمات کلیدی
AEBSF4-(2-aminoethyl)benzenesulfonyl fluoridecarboxy-H2DCFDA5-chloromethylfluorescein diacetateCMFDAPCTAVDBSOCFTRDTNBTBSNACDMSO - DMSON-acetylcysteine - N-استیل سیستئینROS - ROSbuthionine sulfoximine - بوته یون سولفسیمیمTris-buffered saline - تریس بافر شورapoptotic volume decrease - حجم آپوپتوزی کاهش می یابدDimethyl sulfoxide - دیمتیل سولفواکسیدProximal tubules - لوله های پروگزیمالPatch clamp - پچ گیرKidney - کلیهGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of this study was to characterize the role of CFTR during Cd2+-induced apoptosis. For this purpose primary cultures and cell lines originated from proximal tubules (PCT) of wild-type cftr+/+ and cftrâ/â mice were used. In cftr+/+ cells, the application of Cd2+ (5 μM) stimulated within 8 min an ERK1/2-activated CFTR-like Clâ conductance sensitive to CFTRinh-172. Thereafter Cd2+ induced an apoptotic volume decrease (AVD) within 6 h followed by caspase-3 activation and apoptosis. The early increase in CFTR conductance was followed by the activation of volume-sensitive outwardly rectifying (VSOR) Clâ and TASK2 K+ conductances. By contrast, cftrâ/â cells exposed to Cd2+ were unable to develop VSOR currents, caspase-3 activity, and AVD process and underwent necrosis. Moreover in cftr+/+ cells, Cd2+ enhanced reactive oxygen species (ROS) production and induced a 50% decrease in total glutathione content (major ROS scavenger in PCT). ROS generation and glutathione decrease depended on the presence of CFTR, since they did not occur in the presence of CFTRinh-172 or in cftrâ/â cells. Additionally, Cd2+ exposure accelerates effluxes of fluorescent glutathione S-conjugate in cftr+/+ cells. Our data suggest that CFTR could modulate ROS levels to ensure apoptosis during Cd2+ exposure by modulating the intracellular content of glutathione.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 46, Issue 8, 15 April 2009, Pages 1017-1031
Journal: Free Radical Biology and Medicine - Volume 46, Issue 8, 15 April 2009, Pages 1017-1031
نویسندگان
Sebastien L'hoste, Abderrahmen Chargui, Radia Belfodil, Christophe Duranton, Isabelle Rubera, Baharia Mograbi, Chantal Poujeol, Michel Tauc, Philippe Poujeol,