کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10738858 | 1046838 | 2005 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The expression of the NADPH oxidase subunit p22phox is regulated by a redox-sensitive pathway in endothelial cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DMEMECFPDcfHBSSCM-H2DCFDADPIDHR5-bromo-2′-deoxyuridine - 5-bromo-2'-deoxyuridineERK1/2 - ERK1 / 2l-NAME - L-NAMEMAPK - MAPKDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccop38 MAPK - P38 MAPKBrdU - بروموداکسی اوریدینdihydrorhodamine-123 - دی هیدرو رودامین 123dihydroethidium - دی هیدروتیدیمDiphenyleneiodonium - دیفنیلن اندونیمHanks' balanced salt solution - محلول نمک متعادل هانکسnitro-l-arginine methyl ester - نیترو-ال-آرژینین متیل استرhypoxanthine, aminopterin, thymidine - هیپوکسانتین، آمینوپترین، تیمیدینDHE - وmitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استp38 mitogen activated protein kinase - پروتئین کیناز فعال میتوکندر P38HAT - کلاهextracellular regulated kinase - کیناز تنظیم شده خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The expression of the NADPH oxidase subunit p22phox is regulated by a redox-sensitive pathway in endothelial cells The expression of the NADPH oxidase subunit p22phox is regulated by a redox-sensitive pathway in endothelial cells](/preview/png/10738858.png)
چکیده انگلیسی
Endothelial dysfunction is characterized by increased levels of reactive oxygen species (ROS) and a prothrombotic state. The mechanisms linking thrombosis to ROS production in the endothelium are not well understood. We investigated the role of thrombin in regulating NADPH oxidase-dependent ROS production and expression of its subunit p22phox in the endothelial cell line EaHy926. Thrombin elicited a biphasic increase in ROS generation peaking within 15 min, but also at 3 h. The delayed response was accompanied by increased p22phox mRNA and protein expression. Two-photon confocal laser microscopy showed colocalization between p22phox and ROS production. Antioxidant treatment with vitamin C or diphenyleneiodonium abrogated thrombin-induced ROS production and p22phox expression, whereas H2O2 elevated ROS production and p22phox levels. Both responses were dependent on p38 MAP kinase and phosphatidylinositol-3-kinase (PI3 kinase)/Akt. Finally, p22phox was required for thrombin- or H2O2-stimulated proliferation. These data show that thrombin rapidly increases ROS production in endothelial cells, resulting, via activation of p38 MAP kinase and PI3 kinase/Akt, in upregulation of p22phox accompanied by a delayed increase in ROS generation and enhanced proliferation. These findings suggest a positive feedback mechanism whereby ROS, possibly generated by the NADPH oxidase, lead to elevated levels of p22phox and, thus, sustained ROS generation as is observed in endothelial dysfunction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 5, 1 March 2005, Pages 616-630
Journal: Free Radical Biology and Medicine - Volume 38, Issue 5, 1 March 2005, Pages 616-630
نویسندگان
Talija Djordjevic, Alexej Pogrebniak, Rachida S. BelAiba, Steve Bonello, Christoph Wotzlaw, Helmut Acker, John Hess, Agnes Görlach,