کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739887 | 1046893 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Spectroscopic properties and reactivity of free radical forms of A2E
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
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چکیده انگلیسی
A pyridinium bisretinoid (A2E) is the only identified blue-absorbing chromophore of retinal lipofuscin that has been linked to its aerobic photoreactivity and phototoxicity. Pulse radiolysis has been used to study both the one-electron oxidation and the one-electron reduction of A2E in aqueous micellar solutions. The reduction to the semireduced A2E (λmax broad and between 500 and 540 nm) was achieved with formate radicals and the subsequent decay of A2E was slow (over hundreds of milliseconds) via complex kinetics. The long lifetime of the A2E should facilitate its reactions with other biomolecules. For example, with oxygen, the A2E produced the superoxide radical anion with a rate constant of 3 à 108 Mâ1 sâ1. The A2E was also reduced by the NAD radical, the corresponding rate constant being 2.3 à 108 Mâ1 sâ1. Other experiments showed that the one-electron reduction potential of A2E lies in the range â640 to â940 mV. The semioxidized form of A2E (λmax 590 nm) was formed via oxidation with the Br2â radical and had a much shorter lifetime than the semireduced form. With strongly oxidizing peroxyl radicals (CCl3O2) our kinetic data suggest the formation of a radical adduct followed by dissociation to the semioxidized A2E. With milder oxidizing peroxyl radicals such as that from methanol, our results were inconclusive. In benzene we observed an efficient oxidation of zeaxanthin to its radical cation by the A2E radical cation; this may be relevant to a detrimental effect of A2E in vision.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 8, 15 April 2005, Pages 1037-1046
Journal: Free Radical Biology and Medicine - Volume 38, Issue 8, 15 April 2005, Pages 1037-1046
نویسندگان
Agnieszka Broniec, Anna Pawlak, Tadeusz Sarna, Albert Wielgus, Joan E. Roberts, Edward J. Land, T. George Truscott, Ruth Edge, Suppiah Navaratnam,