کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10739892 | 1046893 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Arginase-1 overexpression induces cationic amino acid transporter-1 in psoriasis
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کلمات کلیدی
CATRT-PCRiNOScNOSArginase - آرژینازl-arginine - آرژینین الcationic amino acid transporter - حمل و نقل اسید آمینه اسید کاتیونیFree radicals - رادیکال آزادinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییconstitutive nitric oxide synthase - سنتاز اکسید نیتریک پایدارNitric oxide - نیتریک اکسیدPsoriasis - پسوریازیس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Arginase-1 overexpression induces cationic amino acid transporter-1 in psoriasis Arginase-1 overexpression induces cationic amino acid transporter-1 in psoriasis](/preview/png/10739892.png)
چکیده انگلیسی
Regulated uptake of extracellular l-arginine by cationic amino acid transporters (CATs) is required for inducible nitric oxide synthase and arginase activity. Both enzymes were recently recognized as important in the pathophysiology of psoriasis because of their contribution to epidermal hyperproliferation. We here characterize the expression pattern of CATs in psoriatic skin compared to healthy skin. CAT-1 mRNA expression was strongly upregulated in lesional and nonlesional areas of psoriatic skin compared to healthy skin, whereas expression of CAT-2A and the inducible isoform CAT-2B was unaltered in psoriatic skin. Furthermore, we tested the hypothesis that arginase-1 overexpression regulates CAT expression via intracellular l-arginine concentration. In in vitro experiments with arginase-1 overexpressing HaCaT cells, CAT-1 mRNA expression was increased. Likewise, this occurs in l-arginine-starved HaCaT cells. Both CAT-2 isoforms were not affected. Arginase-1 overexpression limits the synthesis of NO at physiological, but not supraphysiological, l-arginine levels. Plasma l-arginine concentration was diminished in psoriasis patients and the arginase product l-ornithine was significantly increased compared to healthy controls. In summary, arginase-1 overexpression leads to upregulated CAT-1 expression in psoriatic skin, which is due to lowered intracellular l-arginine levels and limits NO synthesis at physiological l-arginine concentrations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 38, Issue 8, 15 April 2005, Pages 1073-1079
Journal: Free Radical Biology and Medicine - Volume 38, Issue 8, 15 April 2005, Pages 1073-1079
نویسندگان
Oliver Schnorr, Maximilian Schuier, Guido Kagemann, Ronald Wolf, Markus Walz, Thomas Ruzicka, Ertan Mayatepek, Maurice Laryea, Christoph V. Suschek, Victoria Kolb-Bachofen, Helmut Sies,