کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10747190 | 1048909 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression and characterization of anionic components in the tubulointerstitial compartment of rat kidney during polymicrobial sepsis
ترجمه فارسی عنوان
بیان و مشخص نمودن اجزای آنیونی در بخش توبولونینستریتیک کلیه موش در طی سپسیس پلی میکروبی
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کلمات کلیدی
CLPbHABPGFBGAGsMAAHRPAKIFBSDABBSA - BSAMaackia amurensis agglutinin - Maackia amurensis آگلوتیینینacute kidney injury - آسیب حاد کلیهbovine serum albumin - آلبومین سرم گاوCecal ligation and puncture - انقباض و سوراخ شدن سکته مغزیDistal tubule - تومور دیستالdiaminobenzidine - دیامینو بنزیدینdigoxigenin - دیگوکسین ژنfetal bovine serum - سرم جنین گاوDIG - شماglomerular filtration barrier - مانع فیلتراسیون گلومرولیHyaluronan - هیالورونانHorseradish peroxidase - پراکسیداز هوررادیشGlycosaminoglycans - گلیکوز آمینو گلیکان ها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
The aim of the study was to evaluate sialic acids and hyaluronan expression, anionic components important for the structure and function of the renal tubulointerstitial compartment, in the early stages of sepsis. Two groups of rats were used: (1) sham-operated controls; (2) cecal ligation and puncture (CLP) (polymicrobial sepsis model). A search for microbial growth was made in the peritoneal fluid to document infection. Tubular function was evaluated by means of urinary protein loss, urinary Na+ and urea excretion. Kidney samples were processed to analyze histology, sialic acids (lectin histochemistry) and hyaluronan (immunohistochemistry) expression. Results showed increased urinary protein loss and fractional excretion of Na+ and urea reduction in the CLP group. Histological changes, particularly in the cortex and in proximal tubules of the CLP group, were observed. In septic rats, compared to controls, sialic acids decreased in amount and their acetylation increased in the tubules, although to a lesser extent in the proximal portion. Hyaluronan was expressed in the medullary interstitium and in a few areas of cortex in controls. In septic rats it increased in the cortical interstitium and appeared in proximal tubules. These results suggest correlation between expression changes of anionic components and tubulointerstitium morphofunctional alterations during sepsis. A role of these molecules in protection/defense and repair processes may be suggested.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 116, Issue 1, January 2014, Pages 94-105
Journal: Acta Histochemica - Volume 116, Issue 1, January 2014, Pages 94-105
نویسندگان
Chiara Adembri, Valentina Selmi, Luca Vitali, Daniele Nosi, Alessia Tani, Giorgia Donata Zappoli Thyrion, Giovanna Santoro, Laura Bonaccini, Gianfranco Avveduto, Anna Lucia Caldini, Eleonora Sgambati,