کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10747229 | 1048914 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Connexin36 (Cx36) expression and protein detection in the mouse carotid body and myenteric plexus
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کلمات کلیدی
PBSconnexinsCx36GFAPDTTRT-PCRgap junctions - اتصالات شکاف دارMyenteric plexus - التهاب مزانترCxs - تحت محاصرهcarotid body - جسم کاروتید، جسم سباتیdithiothreitol - دیتیوتریتولreverse transcription PCR - رونویسی معکوس PCRPhosphate buffered saline - فسفات بافر شورMouse - موشpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالconnexin - کنسکسین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Connexin36 (Cx36) expression and protein detection in the mouse carotid body and myenteric plexus Connexin36 (Cx36) expression and protein detection in the mouse carotid body and myenteric plexus](/preview/png/10747229.png)
چکیده انگلیسی
Although connexin36 (Cx36) has been studied in several tissues, it is notable that no data are available on Cx36 expression in the carotid body and the intestine. The present study was undertaken to evaluate using immunohistochemistry, PCR and Western blotting procedures, whether Cx36 was expressed in the mouse carotid body and in the intestine at ileum and colon level. In the carotid body, Cx36 was detected as diffuse punctate immunostaining and as protein by Western blotting and mRNA by RT-PCR. Cx36 punctate immunostaining was also evident in the intestine with localization restricted to the myenteric plexus of both the ileum and the colon, and this detection was also confirmed by Western blotting and RT-PCR. All the data obtained were validated using Cx36 knockout mice. Taken together the present data on localization of Cx36 gap-junctions in two tissues of neural crest-derived neuroendocrine organs may provide an anatomical basis for future functional investigations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 115, Issue 3, April 2013, Pages 252-256
Journal: Acta Histochemica - Volume 115, Issue 3, April 2013, Pages 252-256
نویسندگان
Monica Frinchi, Valentina Di Liberto, Sada Turimella, Francesca D'Antoni, Martin Theis, Natale Belluardo, Giuseppa Mudò,