Histochemical studies on mucin-rich cells in the digestive tract of a teleost, the Buenos Aires tetra (Hyphessobrycon anisitsi)

Types of mucus cells and mucins in the epithelial cell layer throughout the digestive tract of the Buenos Aires tetra (Hyphessobrycon anisitsi) are described and compared. The mucin was produced in three different cell types: in sac-like cells in the esophagus, in surface epithelial cells in the stomach and in goblet cells in the caeca and intestine. Nearly the entire esophageal epithelial cell layer consisted of mucus cells, filled by both neutral mucin and non-sulfated acidic mucin. The gastric mucin occurred in the distal area of the surface epithelial cells only and contained mainly neutral proteoglycans rich in glucosamine and some galactosamine and sialic acid. The goblet cells contained mainly non-sulfated acidic mucin in the caeca and sulfated acidic mucin throughout the entire intestine. Much glucosamine and some galactosamine and sialic acid occurred regularly in these cells in both the caeca and intestine. The observation that goblet cells often displayed colors ranging between blue and purple-magenta when alcian blue staining was followed by periodic acid-Schiff (PAS), or between blue and red-brown when the alcian blue was followed by neutral red, may reflect different ages or stages of development and differentiation for these cells. The highly variable affinities to wheat germ agglutinin (WGA-lectin) seen in these cells in the present study strengthens this view. However, such results may also suggest a true cellular heterogeneity reflecting various roles in lubrication, immunological defence, digestion and absorption.