کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10818877 1060271 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and localisation of selected myotropic neuropeptides in the ventral nerve cord of tenebrionid beetles
ترجمه فارسی عنوان
شناسایی و جایگزینی نوروپپتید های میتوپنی انتخاب شده در بند ناف عصبی ساطع سوسک های تانریونید
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
MALDI-TOF MS and MS/MS techniques were used for the isolation and identification of neuropeptides from the ventral nerve cord (VNC) of two beetle species Tenebrio molitor and Zophobas atratus. Two peptides, proctolin and myosuppressin (Zopat-MS), with well-established myotropic properties were identified as well as Trica-NVPL-4trunc. The presence of proctolin and myosuppressin was confirmed by immunocytochemical studies in adults and larvae of both beetles. In addition, the myosuppressin gene in Z. atratus was sequenced and expression analyses showed that it is present in all parts of the beetle central nervous system. Results suggest that the identified peptides act as neurotransmitters/neuromodulators in beetles, regulate visceral muscle contractions and indirectly influence important physiological processes such as feeding and reproduction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 166, Issue 1, September 2013, Pages 44-51
نویسندگان
, , , , ,