کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10818992 | 1060291 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular ontogenesis of digestive capability and associated endocrine control in Atlantic cod (Gadus morhua) larvae
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کلمات کلیدی
CARTBile salt activated lipaseDPHACADMPLA2CCKBALNPYQuantitative PCR - PCR کمیphospholipase A2 - آنزیم فسفولیپاز A2 Ontogeny - انتگرالOrexin - اورکسینdays post-hatch - روز بعد از اخراجAtlantic cod larvae - لاروهای ساحلی آتلانتیکGrowth hormone - هورمون رشدPreprosomatostatin - پیشزوماتوستاتینcholecystokinin - کولهسیستوکینینDigestion - گوارش یا هضم یا تحلیلthyroid hormone receptor - گیرنده هورمون تیروئیدNeuropeptide Y - یوروپروتئین Y
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular ontogenesis of digestive capability and associated endocrine control in Atlantic cod (Gadus morhua) larvae Molecular ontogenesis of digestive capability and associated endocrine control in Atlantic cod (Gadus morhua) larvae](/preview/png/10818992.png)
چکیده انگلیسی
We have profiled the expression of twelve genes, in order to provide an overview on the molecular ontogeny of digestive capability with the associated endocrine control during Atlantic cod (Gadus morhua) larval development. Enzyme activity levels for the key digestive enzyme, trypsin, was also measured. Specifically, transcripts for trypsin, amylase, lipolytic enzymes: bile salt activated lipase (BAL), phospholipase A2 (PLA2) and Acyl CoA dehydrogenase (ACADM), regulatory peptides: neuropeptide Y (NPY), orexin (OX) cholecystokinin (CCK) and cocaine and amphetamine-related transcript (CART), the somatotropic factors: growth hormone (GH), preprosomatostatin 1 (PPSS1) and thyroid hormone receptors (TRα and TRβ) were analyzed using quatitative (real-time) polymerase chain reaction (qPCR). Trypsin and BAL mRNA levels peaked at approximately day 17 and 25 post-hatch, respectively, and thereafter displayed a decreasing pattern until metamorphosis. GH mRNA levels decreased moderately from 3 to 33 dph, and thereafter, an increase was observed until 46 dph. TRα mRNA levels showed a fluctuating pattern peaking at day 39 post-hatch. TRβ mRNA levels were too low to obtain quantitative measurements. Amylase mRNA slightly increased from day 3 to 17 post-hatch, and thereafter showed a steady decrease until day 60. Interestingly, PLA2 mRNA expression showed a consistent increase throughout the study period, indicating an increasingly important role during larval development. Overall, data from this study indicate that cod larvae show differential developmental mode of expression patterns for key genes and endocrine factors that regulate digestive capability, growth and development. These data are discussed in relation to larval trypsin enzyme activity and previous reports for other teleost species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 160, Issue 2, October 2011, Pages 190-199
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 160, Issue 2, October 2011, Pages 190-199
نویسندگان
Trond M. Kortner, Ingrid Overrein, Gunvor Ãie, Elin Kjørsvik, Tora Bardal, Per-Arvid Wold, Augustine Arukwe,