کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10821778 | 1061541 | 2005 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vitamin E prevents cell death induced by mild oxidative stress in chicken skeletal muscle cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
EDDnpABZCHXECLDAPIGSHDTTHEPESDMEM3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate - 3 - [(3-کلامیدوپروپیل) دی متیل آمونیو] -1-پروپان سولفونات4′-6-diamidino-2-phenylindole - 4'-6-diamidino-2-phenylindoleBSA - BSADulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoHydrogen peroxide - آب اکسیژنهbovine serum albumin - آلبومین سرم گاوEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدAminobenzoic acid - اسید امینوبنزوئیکdithiothreitol - دیتیوتریتولcycloheximide - سیکلوهایسیمیدH2O2 - هیدروژن پراکسیدCHAPS - چاپسreduced glutathione - کاهش گلوتاتیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Vitamin E prevents cell death induced by mild oxidative stress in chicken skeletal muscle cells Vitamin E prevents cell death induced by mild oxidative stress in chicken skeletal muscle cells](/preview/png/10821778.png)
چکیده انگلیسی
Apoptosis and necrosis are two forms of cell death that can occur in response to various agents and oxidative damage. In addition to necrosis, apoptosis contributes to muscle fiber loss in various muscular dystrophies as well participates in the exudative diathesis in chicken, pathology caused by dietary deficiency of vitamin E and selenium, which affects muscle tissue. We have used chicken skeletal muscle cells and bovine fibroblasts to study molecular events involved in the cell death induced by oxidative stress and apoptotic agents. The effect of vitamin E on cell death induced by oxidants was also investigated. Treatment of cells with anti-Fas antibody (50 to 400 ng/mL), staurosporine (0.1 to 100 μM) and TNF-α (10 and 50 ng/mL) resulted in a little loss of Trypan blue exclusion ability. Those stimuli conducted cells to apoptosis detected by an enhancement in caspase activity upon fluorogenic substrates but this activity was not fully blocked by the caspase inhibitor Z-VAD-fmk. Oxidative stress induced by menadione (10, 100 and 250 μM) promoted a significant reduction in cell viability (10%, 20% and 35% for fibroblasts; 20%, 30% and 75% for muscle cells, respectively) and caused an increase in caspase activity and DNA fragmentation. H2O2 also promoted apoptosis verified by caspase activation and DNA fragmentation, but in higher doses induced necrosis. Vitamin E protected cells from death induced by low doses of oxidants. Although it was ineffective in reducing caspase activity in fibroblasts, this vitamin diminished the enzyme activity in muscle cells. These data suggested that oxidative stress could activate apoptotic mechanisms; however the mode of cell death will depend on the intensity and duration of the stimulus, and on the antioxidant status of the cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 141, Issue 3, July 2005, Pages 225-240
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 141, Issue 3, July 2005, Pages 225-240
نویسندگان
Viviane A. Nunes, Andrezza J. Gozzo, Ilana Cruz-Silva, Maria A. Juliano, Tania A. Viel, Rosely O. Godinho, Flavio V. Meirelles, Misako U. Sampaio, Claudio A.M. Sampaio, Mariana S. Araujo,