A triple combination strategy corrects population stratification bias and saves genotyping cost

ObjectiveThe case-control association study has gained popularity for mapping disease-susceptibility gene(s) of complex human diseases. However, the study is prone to population stratification bias.Study Design and SettingThe authors proposed a triple combination strategy of stratum matching, genomic control, and multiple DNA pooling for population-based case-control association studies. Monte Carlo simulation showed that the type I error rates are well controlled using this strategy.ResultsAs for power, it increases under the following situations: (1) more accurate stratum matching, (2) more null markers for genomic control, (3) more DNA pooling sets, and (4) more accurate DNA pooling measurement.ConclusionThe triple combination strategy corrects population stratification bias and saves genotyping cost.