Development of an analytical approach for identification and quantification of 5-α-androst-16-en-3-one in human milk

► We developed a method for the quantification of androstenone in human milk. ► The quantification of androstenone in human milk was performed by stable isotope dilution assay. ► Concentrations of androstenone in human milk samples ranged from 26 to 155 ng/kg.