کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10848137 | 1070654 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MRI quantification in vivo of corticosteroid induced thymus involution in mice: Correlation with ex vivo measurements
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کلمات کلیدی
DEXNFκB - NFKBMRI - امآرآی یا تصویرسازی تشدید مغناطیسیInvolution - انفجارMagnetic resonance - تشدید مغناطیسیMagnetic resonance imaging - تصویربرداری رزونانس مغناطیسیThymus - تیموسDexamethasone - دگزامتازونnuclear factor kappa-B - فاکتور هسته ای کاپا-BMouse - موشactivator protein-1 - پروتئین فعال کننده-1Corticosteroid - کورتیکواستروئیدها
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Thymus involution is a useful marker of transactivation-mediated side effects in preclinical therapeutic index testing of new anti-inflammatory glucocorticosteroids, and is usually measured post mortem. We have validated the use of MRI for non-invasive in vivo measurement of mouse thymus involution induced by dexamethasone (DEX). Tl-weighted spin echo 7 T images provided satisfactory contrast between thymus and surrounding connective tissue and fat. Increasing doses of DEX caused thymus involution, reflected in MRI volume (87 ± 14, 33 ± 10, 28 ± 6, 16 ± 7 μl in dosage groups of Cremophor vehicle, 1, 10 and 30 mg/kg subcutaneous respectively, n = 6/group, mean ± standard deviation) and post mortem wet weight (64 ± 12, 33 ± 6, 25 ± 9, 23 ± 8 mg). Correlation between MRI volumes and wet weights was very good (r = 0.842). Measuring pre-dose MRI volumes and then assessing DEX effects as post-dose change from baseline produced no statistical advantage relative to considering post-dose MRI thymus volume alone, probably due to variability in pre-dose baseline values compounding post-dose variability. Smaller group sizes were sufficient to achieve a given statistical power using MRI post-dose volume than using wet weight, suggesting a role for MRI in differentiating the effects of compounds which produce similar effects, or in contexts where the use of large groups of animals is impractical or ethically unacceptable.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Steroids - Volume 70, Issue 4, April 2005, Pages 267-272
Journal: Steroids - Volume 70, Issue 4, April 2005, Pages 267-272
نویسندگان
Keith J. Brooks, Keith T. Bunce, Michael V. Haase, Alan White, K. Kumar Changani, Simon T. Bate, David G. Reid,