کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10852670 | 1071982 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro innate immune cell based models to assess whole cell Bordetella pertussis vaccine quality: A proof of principle
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کلمات کلیدی
LPSMFIPAMAPCPRRTregPam3CSK4MoDCAntigen presenting cells - آنتیژن ارائه سلولEscherichia coli - اشریشیا کُلیstandard deviation - انحراف معیارInnate immunity - ایمنی ذاتیE. coli - باکتری E. coliBordetella pertussis - بوردتلا پرتوزیسIn vitro - در فضای بازmonocyte derived dendritic cells - سلولهای دندریتیک مونوسیت مونوسیتRegulatory T cells - سلولهای تی تنظیمکنندهlipopolysaccharide - لیپوپلی ساکاریدmean fluorescence intensity - میانگین شدت فلورسانسVaccines - واکسنpathogen recognition receptor - گیرنده شناسایی پاتوژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
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چکیده انگلیسی
Lot release testing of vaccines is primarily based on animal models that are costly, time-consuming and sometimes of questionable relevance. In order to reduce animal use, functional in vitro assays are being explored as an alternative approach for the current lot release testing paradigm. In this study, we present an evaluation of APC platforms assessing innate immune activation by whole cell Bordetella pertussis (wP) vaccines. Primary monocytes, monocyte-derived DC (moDC) and human monocyte/DC cell lines (MonoMac6 and MUTZ-3) were compared for their capacity to respond to wP vaccines of varying quality. To produce such vaccines, the production process of wP was manipulated, resulting in wP vaccines covering a range of in vivo potencies. The responses of MUTZ-3 cells and primary monocytes to these vaccines were marginal and these models were therefore considered inappropriate. Importantly, moDC and MonoMac6 cells responded to the wP vaccines and discriminated between vaccines of varying quality, although slight variations in the responses to wP vaccines of similar quality were also observed. This study provides a proof of principle for the use of in vitro APC platforms as part of a new strategy to assess wP vaccine lot consistency, though careful standardisation of assay conditions is necessary.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 43, Issue 2, March 2015, Pages 100-109
Journal: Biologicals - Volume 43, Issue 2, March 2015, Pages 100-109
نویسندگان
M.E. Hoonakker, L.M. Verhagen, C.F.M. Hendriksen, C.A.C.M. van Els, R.J. Vandebriel, A. Sloots, W.G.H. Han,