کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10870047 1073987 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
GBF/Gea mutant with a single substitution sustains fungal growth in the absence of BIG/Sec7
چکیده انگلیسی
Golgi Arf1-guanine nucleotide exchange factors (GEFs) belong to two subfamilies: GBF/Gea and BIG/Sec7. Both are conserved across eukaryotes, but the physiological role of each is not well understood. Aspergillus nidulans has a single member of the early Golgi GBF/Gea-subfamily, geaA, and the late Golgi BIG/Sec7-subfamily, hypB. Both geaA and hypB are essential. hypB5 conditionally blocks secretion. We sought extragenic hypB5 suppressors and obtained geaA1. geaA1 results in Tyr1022Cys within a conserved GBF/Gea-specific S(Y/W/F)(L/I) motif in GeaA. This mutation alters GeaA localization. Remarkably, geaA1 suppresses hypBΔ, indicating that a single mutant Golgi Arf1-GEF suffices for growth.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 588, Issue 24, 20 December 2014, Pages 4799-4806
نویسندگان
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