کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10870252 1073995 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functional response of the small multidrug resistance protein EmrE to mutations in transmembrane helix 2
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Functional response of the small multidrug resistance protein EmrE to mutations in transmembrane helix 2
چکیده انگلیسی
Escherichia coli EmrE is a small multidrug resistance protein encompassing four transmembrane (TM) sequences that oligomerizes to confer resistance to antimicrobials. Here we examined the effects on in vivo protein accumulation and ethidium resistance activity of single residue substitutions at conserved and variable positions in EmrE transmembrane segment 2 (TM2). We found that activity was reduced when conserved residues localized to one TM2 surface were replaced. Our findings suggest that conserved TM2 positions tolerate greater residue diversity than conserved sites in other EmrE TM sequences, potentially reflecting a source of substrate polyspecificity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 588, Issue 20, 16 October 2014, Pages 3720-3725
نویسندگان
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