کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10870399 | 1074000 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Roles of intraloops-2 and -3 and the proximal C-terminus in signalling pathway selection from the human calcium-sensing receptor
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کلمات کلیدی
GPCRCaSRVFTPI-PLCIP1inositol 1-phosphatecAMP - cAMPERK1/2 - ERK1 / 2G-protein coupled receptor - G-پروتئین همراه گیرندهadenosine 3′,5′-cyclic monophosphate - آدنوزین 3 '، 5'-سیکلیک منوفسفرهBiased signalling - سیگنال منصفانهCysteine-rich - غنی از سیتینVenus flytrap - پرواز ونوسPERK - پرکCalcium-sensing receptor - گیرنده سنجش کلسیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Roles of intraloops-2 and -3 and the proximal C-terminus in signalling pathway selection from the human calcium-sensing receptor Roles of intraloops-2 and -3 and the proximal C-terminus in signalling pathway selection from the human calcium-sensing receptor](/preview/png/10870399.png)
چکیده انگلیسی
The calcium-sensing receptor (CaSR) couples to signalling pathways via intracellular loops 2 and 3, and the C-terminus. However, the requirements for signalling are largely undefined. We investigated the impacts of selected point mutations in iL-2 (F706A) and iL-3 (L797A and E803A), and a truncation of the C-terminus (R866X) on extracellular Ca2+ (Ca2+o)-stimulated phosphatidylinositol-specific phospholipase-C (PI-PLC) and various other signalling responses. CaSR-mediated activation of PI-PLC was markedly attenuated in all four mutants and similar suppressions were observed for Ca2+o-stimulated ERK1/2 phosphorylation. Ca2+o-stimulated intracellular Ca2+ (Ca2+i) mobilization, however, was relatively preserved for the iL-2 and iL-3 mutants and suppression of adenylyl cyclase was unaffected by either E803A or R866X. The CaSR selects for specific signalling pathways via the proximal C-terminus and key residues in iL-2, iL-3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 588, Issue 18, 17 September 2014, Pages 3340-3346
Journal: FEBS Letters - Volume 588, Issue 18, 17 September 2014, Pages 3340-3346
نویسندگان
Mahvash A. Goolam, James H. Ward, Vimesh A. Avlani, Katie Leach, Arthur Christopoulos, Arthur D. Conigrave,