کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10870749 | 1074019 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Antifreeze activity enhancement by site directed mutagenesis on an antifreeze protein from the beetle Rhagium mordax
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Antifreeze activity enhancement by site directed mutagenesis on an antifreeze protein from the beetle Rhagium mordax Antifreeze activity enhancement by site directed mutagenesis on an antifreeze protein from the beetle Rhagium mordax](/preview/png/10870749.png)
چکیده انگلیسی
The ice binding motifs of insect antifreeze proteins (AFPs) mainly consist of repetitive TxT motifs aligned on a flat face of the protein. However, these motifs often contain non-threonines that disrupt the TxT pattern. We substituted two such disruptive amino acids located in the ice binding face of an AFP from Rhagium mordax with threonine. Furthermore, a mutant with an extra ice facing TxT motif was constructed. These mutants showed enhanced antifreeze activity compared to the wild type at low concentrations. However, extrapolating the data indicates that the wild type will become the most active at concentrations above 270 μmol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 588, Issue 9, 2 May 2014, Pages 1767-1772
Journal: FEBS Letters - Volume 588, Issue 9, 2 May 2014, Pages 1767-1772
نویسندگان
Dennis Steven Friis, Erlend Kristiansen, Nicolas von Solms, Hans Ramløv,