کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10870796 1074020 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
FixL-like sensor FlbS of Brucella abortus binds haem and is necessary for survival within eukaryotic cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
FixL-like sensor FlbS of Brucella abortus binds haem and is necessary for survival within eukaryotic cells
چکیده انگلیسی
Replication of Brucella inside eukaryotic cells is essential for pathogenesis, and successful infection requires rapid adaptation to the intracellular milieu. Close relatives of Brucella use the two-component system FixLJ to survive inside the host. We aimed to identify a homologous sensor in Brucella abortus. A predicted protein with transmembrane and conserved histidine kinase domains was identified as the Fix-like Brucella sensor, FlbS. Although it lacks the PAS domain, recombinant FlbS binds haem in vitro. An internal in-frame deletion in flbS severely decreased B. abortus survival inside professional and non-professional phagocytes. This phenotype was reverted by genetic complementation. These results indicate the critical role of this haemoprotein in the intracellular lifestyle of Brucella.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 587, Issue 18, 17 September 2013, Pages 3102-3107
نویسندگان
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