Cdk1 interplays with Oct4 to repress differentiation of embryonic stem cells into trophectoderm

► Oct4 can directly interact with Cdk1 in vitro and in vivo. ► Cdk1 enhances the binding of Oct4 on trophectoderm marker, Cdx2 and promotes Cdx2 repression in mouse embryonic stem cells. ► The role of Cdk1 in regulating Oct4-mediated Cdx2 repression is independent of Cyclins and its kinase activity.