Glucose-dependent regulation of AMP-activated protein kinase in MIN6 beta cells is not affected by the protein kinase A pathway

► Glucose induces phosphorylation of AMPKα1 at Ser485 and Ser497. ► Phosphorylation of AMPKα1 at Thr172 and Ser485/497 follows an inverse correlation. ► Phosphorylation of AMPKα1 by PKA does not impair phosphorylation of Thr172. ► Phosphorylation of AMPKα1 at Thr172 does not affect phosphorylation by PKA. ► Phosphorylation of Thr172 and Ser485/497 follows an independent regulation.