4E-BP3 regulates eIF4E-mediated nuclear mRNA export and interacts with replication protein A2

► We found 4E-BP3 acts as an inhibitor of eIF4E-mediated mRNA export. ► 4E-BP3 interacts with dephosphorylated form of RPA2, and could not interact with phosphorylated form of RPA2. ► The inhibition ability of 4E-BP3 on eIF4E-mediated mRNA export is regulated by the phosphorylation state of RPA2.