Oxo-phytodienoic acid (OPDA) is formed on fatty acids esterified to galactolipids after tissue disruption in Arabidopsis thaliana

► We examined accumulation of OPDA containing galactolipids in Arabidopsis. ► Freeze-thawing caused massive accumulation. ► The lipid profile was examined. ► The biosynthesis pathway was examined by stable isotope labeling. ► OPDA containing lipids are produced without release of free fatty acids.