A conserved regulatory element located far downstream of the gls locus modulates gls expression through chromatin loop formation during myogenesis

► A distant regulatory element is located 120 kb downstream of the gls promoter. ► The element enhances the transcriptional activities of promoters. ► The element physically interacts with the gls promoter. ► The element differentially regulates gls gene transcription during myogenesis. ► The element modulates gene expression through spatial organization of chromatin.