cGMP and cyclic nucleotide-gated channels participate in mouse sperm capacitation

► Capacitation and cyclic nucleotides increase the macroscopic current in mouse sperm. ► There are subpopulations of sperm depending on their response to cyclic nucleotides. ► CNG channels are physiologically active in the mature mouse sperm. ► Sperm CNG channels have different sensitivity between cGMP and cAMP. ► CNG channels participate in sperm capacitation providing an entry pathway for Ca2+.