Double-stranded RNA induces S100 gene expression by a cycloheximide-sensitive factor

► S100A8/A9 gene induction in keratinocytes by TLR3 ligand polyI:C. ► Confirmation in organotypic epithelial “raft” culture after herpes simplex virus infection. ► S100A8/A9 gene induction is not dependent on IL-10. ► Involvement of NF-κB, p38 MAPK, PI-3K and Jak-Stat signal transduction pathways in polyI:C-induced S100A8/A9 gene expression. ► S100A8/A9 gene induction is abrogated by treatment with either cycloheximide or bleomycin.