PKA-dependent regulation of Cdc25 RasGEF localization in budding yeast

► Saccharomyces cerevisiae RasGEF Cdc25 is localized in the nucleus, ER and plasma membrane. ► PKA activity drives Cdc25 to the cytosol. ► Ser825 and Ser826 mutation to Glu impairs Cdc25 localization to the nucleus.