کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10872020 1074095 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthetic peptides identify a second periplasmic site for the plug of the SecYEG protein translocation complex
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Synthetic peptides identify a second periplasmic site for the plug of the SecYEG protein translocation complex
چکیده انگلیسی
A short helix in the centre of the SecY subunit serves as a 'plug' blocking the protein channel. This site must be vacated if the channel is to open and accommodate translocating protein. We have synthesised a peptide mimic of this plug, and show that it binds to E. coli SecYEG, identifying a distinct and peripheral binding site. We propose that during active translocation the plug moves to this second discrete site and chart its position. Deletion of the plug in SecY increases the stoichiometry of the peptide-SecYEG interaction by also exposing the location it occupies in the channel. Binding of the plug peptide to the channel is unaffected by SecA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 1, 5 January 2009, Pages 207-212
نویسندگان
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