Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli

► We compared protein splicing between 20 different inteins in three identical foreign extein contexts. ► Effects of exteins and temperature were compared for the 20 inteins. ► Pho RadA intein and Npu DnaB mini-intein were discovered to have high splicing efficiency.