Keywords: اینتین; Intein; Uricase; Purification; Aspergillus flavus; pTXB;
مقالات ISI اینتین (ترجمه نشده)
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Keywords: اینتین; Ligation-independent cloning; Molecular cloning; Intein; Protein purification; Self-cleaving affinity tag;
Using self-cleavable ternary fusion pattern for efficient preparation of Bacteriorhodopsin
Keywords: اینتین; Self-cleavable; Intein; Expression; Membrane protein; Bacteriorhodopsin;
Pilot production of the recombinant peptide toxin of Heteractis crispa as a potential analgesic by intein-mediated technology
Keywords: اینتین; Recombinant protein; Fusion protein; Intein; Analgesic polypeptide; Toxin;
Expression, purification, and characterization of recombinant 8Â kDa gelsolin fragment
Keywords: اینتین; 8 kDa-GSN; Intein; Amyloidogenic; Expression; Purification; Characterization; CD; circular dichroism; C68; 68 kDa GSN fragment; DLS; dynamic light scattering; DTT; dithiothreitol; E. coli; Escherichia coli; FAF; familial amyloidosis of Finnish; GSN; gel
Assessing activity of Hepatitis A virus 3C protease using a cyclized luciferase-based biosensor
Keywords: اینتین; Firefly luciferase; Biosensor; Hepatitis A virus; 3C protease; Intein;
Production of a polar fish antimicrobial peptide in Escherichia coli using an ELP-intein tag
Keywords: اینتین; ELP; Intein; Antimicrobial peptide; Batch production;
Npro fusion technology: On-column complementation to improve efficiency in biopharmaceutical production
Keywords: اینتین; Recombinant protein production; Autoprotease Npro; Intein; Protein fragment complementation; Immobilization;
Development of the intein-mediated method for production of recombinant thymosin β4 from the acetylated in vivo fusion protein
Keywords: اینتین; Protein acetylation; Thymosin beta 4; RimJ; RimL; Fusion protein; Intein;
Segmental expression and C-terminal labeling of protein ERp44 through protein trans-splicing
Keywords: اینتین; ERp44; Intein; Protein trans-splicing; Segmental expression; C-terminal labeling
Novel intein-containing DNA specific primers for rapid identification of Candida glabrata using Real-Time PCR assays
Keywords: اینتین; C. glabrata; Intein; Real-Time PCR; Rapid detectionC. glabrata; Intéine; PCR en temps réel; Détection rapide
Expression and purification of soluble HIV-2 viral protein R (Vpr) using a sandwich-fusion protein strategy
Keywords: اینتین; HIV accessory proteins; HIV-2 Vpr; Intein; Sandwich fusion protein; DPC; NMR;
A streamlined method for preparing split intein for NMR study
Keywords: اینتین; Intein; Split intein; Circular permutation; Protein trans-splicing; NMR
Cyclization tag for the detection and facile purification of backbone-cyclized proteins
Keywords: اینتین; Protein backbone cyclization; Intein; Myc tag; Split tag; Cyclization tag
Expressed protein ligation-mediated template protein extension
Keywords: اینتین; Expressed protein ligation; Apolipoprotein A-I; Milano; Protein stability; Intein
Ubiquitin-intein and SUMO2-intein fusion systems for enhanced protein production and purification
Keywords: اینتین; Fusion tags; Ubiquitin; SUMO2; Intein; Recombinant protein purification;
Generation of bioactive recombinant Ancylostoma caninum anticoagulant peptide c2
Keywords: اینتین; Ancylostoma caninum anticoagulant peptide c2; Gene clone; Expression; Purification; Intein;
Cell disruption enhanced the pure EGFP recovery from an EGFP-intein-surface protein production system in recombinant E. coli
Keywords: اینتین; Protein; Purification; Surface display; Intein; Centrifugation; Recombinant DNA
Intein-mediated expression, purification, and characterization of thymosin α1–thymopentin fusion peptide in Escherichia coli
Keywords: اینتین; Thymosin α1–thymopentin; Intein; Self-cleavage; Protein refolding; ESI-MS; Immunoregulatory activity
Split-intein mediated re-assembly of genetically encoded Ca2+ indicators
Keywords: اینتین; Genetically encoded calcium indicators; TN-XL; GCaMP2; Intein; C. elegans;
PRP8 inteins in species of the genus Botrytis and other ascomycetes
Keywords: اینتین; Botrytis; Intein; Horizontal transmission; Genetic footprint
Production of recombinant EGFP via surface display of ice nucleation protein and self-cleavage intein
Keywords: اینتین; Recombinant protein production; Surface display; Ice nucleation protein; Intein; Enhanced green fluorescent protein; Protein purification
Spontaneous C-cleavage of a mini-intein without its conserved N-terminal motif A
Keywords: اینتین; Intein; Protein splicing; Protein cleavage;
Characterisation of the coccolithovirus intein
Keywords: اینتین; Intein; Homing endonuclease; Coccolithovirus; Virus; Marine; Evolution
Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli
Keywords: اینتین; Protein splicing; Trans-splicing; Segmental labeling; Protein ligation; Intein;
Intein-mediated one-step purification of Escherichia coli secreted human antibody fragments
Keywords: اینتین; Intein; Chitin-binding domain; Escherichia coli secretion; Recombinant protein purification; Self-cleaving affinity tag; Disulfide bonds;
Recombinant production of antimicrobial peptides in Escherichia coli: A review
Keywords: اینتین; Antimicrobial peptide; Carrier protein; Chemical cleavage; Enzymatic cleavage; Fusion expression; Intein; LL-37; Thioredoxin; SUMO; Recombinant gene expression;
PRP8 intein in Ajellomycetaceae family pathogens: Sequence analysis, splicing evaluation and homing endonuclease activity
Keywords: اینتین; Ajellomycetaceae; Intein; PRP8; Splicing; Homing endonuclease;
Effects of catalytic site mutations on active expression of phage fused penicillin acylase
Keywords: اینتین; Penicillin G acylase; Intein; Phage display; Directed evolution; Mutagenesis; Post-translational auto-splicing;
Mechanism of protein splicing of the Pyrococcus abyssi lon protease intein
Keywords: اینتین; Protein splicing; Intein; Extein; Pyrococcus abyssi; Lon protease; Homing endonuclease
Expression and purification of moricin CM4 and human β-defensins 4 in Escherichia coli using a new technology
Keywords: اینتین; CM4; HβD4; Elastin-like peptide (ELP); Intein; Inverse transition cycling (ITC); Self-cleavage
Sexual mating of Botrytis cinerea illustrates PRP8 intein HEG activity
Keywords: اینتین; Intein; Homing; Homing endonuclease; Gene conversion; Horizontal transfer; Prp8; Splicing
Expression, purification, phosphorylation and characterization of recombinant human statherin
Keywords: اینتین; Intein; ESI–MS; Golgi-casein kinase; ATR-FTIR
Protein splicing of the three Pyrococcus abyssi ribonucleotide reductase inteins
Keywords: اینتین; Protein splicing; Intein; Extein; Pyrococcus abyssi; Ribonucleotide reductase; Homing endonuclease
Optimization of ELP-intein mediated protein purification by salt substitution
Keywords: اینتین; Protein purification; Intein; Elastin-like polypeptide (ELP); Hofmeister; Self-cleaving tag; Non-chromatographic bioseparation;
Solution structure of DnaE intein from Nostoc punctiforme: Structural basis for the design of a new split intein suitable for site-specific chemical modification
Keywords: اینتین; Intein; Protein splicing; NMR spectroscopy; Protein ligation; Nuclear spin relaxation; Chemical modification; Protein trans-splicing
Soluble expression and one-step purification of a neurotoxin Huwentoxin-I in Escherichia coli
Keywords: اینتین; Huwentoxin-I; Neurotoxin; Soluble expression; Intein; N-VSCC; Whole-cell patch clamp;
Protein splicing: A versatile tool for drug discovery
Keywords: اینتین; Intein; Conditional protein splicing; Split-intein; Gene therapy; SICLOPPS; Cyclic peptides; Antimicrobial drug target; Trans-splicing; EPL
“Splicing up” drug discovery. : Cell-based expression and screening of genetically-encoded libraries of backbone-cyclized polypeptides
Keywords: اینتین; Cyclic peptide; Intein; Cyclotides; Genetically-encoded libraries; Native Chemical Ligation; Expressed Protein Ligation; Trans-peptidation
Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage
Keywords: اینتین; Affinity adsorption; Cellulose-binding module; Intein; Protein purification; Regenerated amorphous cellulose
Minimization of a eukaryotic mini-intein
Keywords: اینتین; Intein; Protein splicing; Penicillium chrysogenum; PRP8 protein; Minimization;
Construction of intein-mediated hGMCSF expression vector and its purification in Pichia pastoris
Keywords: اینتین; Pichia pastoris; Intein; rhGMCSF; Fusion tag purification; Recombinant protein;
Production of recombinant human epidermal growth factor using Ssp dnaB mini-intein system
Keywords: اینتین; Human epidermal growth factor (hEGF); Fusion protein; Intein; Expression in Escherichia coli
Towards the elimination of chromatography in protein purification: Expressing proteins engineered to purify themselves
Keywords: اینتین; Intein; Bioseparations; Self-cleaving; Recombinant protein purification; Elastin-like peptide; Polyhydroxybutyrate; Phasin; Affinity tag methods
Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners
Keywords: اینتین; Antibacterial peptide; ABP-CM4; Fusion partner; GST; Intein; Escherichia coli;
Recombinant expression of twelve evolutionarily diverse subfamily Iα aminotransferases
Keywords: اینتین; Aminotransferase; Pyridoxal phosphate; Intein; Heterologous expression; Substrate specificity; Annotation
Trans-splicing of an artificially split fungal mini-intein
Keywords: اینتین; Intein; Protein splicing; Penicillium chrysogenum; PRP8 protein; Trans-splicing
One-step purification of insoluble hydantoinase overproduced in Escherichia coli
Keywords: اینتین; Hydantoinase; Intein; Affinity tag; Artificial oil body; Oleosin
Sequence requirements for splicing by the Cne PRP8 intein
Keywords: اینتین; Protein splicing; Cne PRP8; Intein; Cryptococcus
Expressed protein ligation using an N-terminal cysteine containing fragment generated in vivo from a pelB fusion protein
Keywords: اینتین; Expressed protein ligation; Intein; Apolipoprotein; Apolipophorin; PelB; Leader peptidase