Ubiquitin-intein and SUMO2-intein fusion systems for enhanced protein production and purification

► Ubiquitin-intein and SUMO2-intein fusion tags were constructed. ► Both tags enhance the expression of eGFP and solubility of eGFP and MMP13. ► Both tags allowed convenient purification of eGFP with high homogeneity. ► Both tags may present viable alternatives for protein production in industry process.