کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2071581 1078760 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protein splicing: A versatile tool for drug discovery
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Protein splicing: A versatile tool for drug discovery
چکیده انگلیسی

The judicious application of intein technologies to biological problems has resulted in powerful tools for biomedical research. Inteins are intervening sequences that excise themselves from precursor proteins and ligate the surrounding sequences. Variations of intein chemistry have been used to create tagless protein purification strategies, specifically label expressed proteins for biochemical assays, design biosensors, produce microarrays, and synthesize cyclic peptide libraries for inhibitor studies. Moreover, recent advances in small molecule triggered protein splicing allow for tunable post-translational control of protein function in vivo. Inteins are now positioned as an essential tool to study the mechanism of disease progression and validate drug candidates. Yet these tiny proteins have more tricks to play. Recent progress in gene therapy and drug targeting suggest a bright future where split inteins mediate in vivo reconstruction of large therapeutic proteins and target drugs to a specified site of action. Inteins are rapidly becoming valuable tools for drug discovery and drug delivery.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Advanced Drug Delivery Reviews - Volume 61, Issue 11, 30 September 2009, Pages 899–907
نویسندگان
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