Insight into interactions of the von-Willebrand-factor-A-like domain 2 with the FNIII-like domain 9 of collagen VII by NMR and SPR

► Cloning and purification of murine type VII collagens vWFA2 domain was successful. ► Isotope labelling and NMR resonance assignment has been done. ► Secondary structure prediction and coupling constants provided structural insights. ► Chemical shift mapping and SPR showed an interaction with the FNIII-like domain 9.