کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10872196 | 1074110 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Diffusion of a membrane protein, Tat subunit Hcf106, is highly restricted within the chloroplast thylakoid network
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Diffusion of a membrane protein, Tat subunit Hcf106, is highly restricted within the chloroplast thylakoid network Diffusion of a membrane protein, Tat subunit Hcf106, is highly restricted within the chloroplast thylakoid network](/preview/png/10872196.png)
چکیده انگلیسی
The thylakoid membrane forms stacked thylakoids interconnected by 'stromal' lamellae. Little is known about the mobility of proteins within this system. We studied a stromal lamellae protein, Hcf106, by targeting an Hcf106-GFP fusion protein to the thylakoids and photobleaching. We find that even small regions fail to recover Hcf106-GFP fluorescence over periods of up to 3Â min after photobleaching. The protein is thus either immobile within the thylakoid membrane, or its diffusion is tightly restricted within distinct regions. Autofluorescence from the photosystem II light-harvesting complex in the granal stacks likewise fails to recover. Integral membrane proteins within both the stromal and granal membranes are therefore highly constrained, possibly forming 'microdomains' that are sharply separated.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 583, Issue 22, 19 November 2009, Pages 3690-3696
Journal: FEBS Letters - Volume 583, Issue 22, 19 November 2009, Pages 3690-3696
نویسندگان
Elina Vladimirou, Michael Li, Cassie P. Aldridge, Lorenzo Frigerio, Markus Kirkilionis, Colin Robinson,