کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10872244 1074111 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Regulation of Trk-dependent potassium transport by the calcineurin pathway involves the Hal5 kinase
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Regulation of Trk-dependent potassium transport by the calcineurin pathway involves the Hal5 kinase
چکیده انگلیسی
The phosphatase calcineurin and the kinases Hal4/Hal5 regulate high-affinity potassium uptake in Saccharomyces cerevisiae through the Trk1 transporter. We demonstrate that calcineurin is necessary for high-affinity potassium uptake even in the absence of Na+ stress. HAL5 expression is induced in response to stress in a calcineurin-dependent manner through a newly identified functional CDRE (nt −195/−189). Lack of calcineurin decreases Hal5 protein levels, although with little effect on Trk1 amounts. However, the growth defect of cnb1 cells at K+-limiting conditions can be rescued in part by overexpression of HAL5, and this mutation further aggravates the potassium requirements of a hal4 strain. This suggests that the control exerted by calcineurin on Hal5 expression may be biologically relevant for Trk1 regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 584, Issue 11, 3 June 2010, Pages 2415-2420
نویسندگان
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