کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10872948 | 1074253 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hypotonic stress induces E-cadherin expression in cultured human keratinocytes
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کلمات کلیدی
EGFRMAP kinase/ERK kinaseHypoosmoticMEK1GAPDHE-cadherin - E-CadherinERK1/2 - ERK1 / 2MAPK - MAPKPKB/AKT - PKB / AKTIST - استinternal standard - استاندارد داخلیOsmotic stress - استرس اسمزیReverse transcriptase - ترانس کریپتاز معکوس یا وارونویسHypotonic - هیپوتونیکProtein kinase B/Akt - پروتئین کیناز B / Aktmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenSkin - پوستKeratinocyte - کراتینوسیتextracellular signal-regulated kinase 1/2 - کیناز 1/2 تنظیم سیگنال خارج سلولیglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Human epidermis marks the interface between internal and external environments with the major task being to maintain body hydration. Alternating exposure of skin to a dry or humid environment is likely to cause changes in the epidermal water gradient resulting in osmotic alterations of epidermal keratinocytes. The present in vitro approach studied the effect of hypotonicity on cell-cell contact. It was demonstrated that hypotonic stress applied to human epithelial cells (HaCaT, A-431) induced upregulation of E-cadherin at both, the protein and mRNA level. 5â²-deletional mutants of the E-cadherin promoter identified an element ranging from â53 to +31 that conveyed strong transactivation under hypotonic stress. In order to define relevant upstream regulators members of the MAP kinase family, the epidermal growth factor receptor (EGFR) and protein kinase B/Akt (PKB/Akt) were investigated. Hypotonic conditions led to a fast activation of ERK1/2, SAPK/JNK, p38, EGFR and PKB/Akt with distinct activation patterns. Experiments using specific inhibitors showed that p38 contributes to the E-cadherin transactivation under hypotonic conditions. Further upstream, adhesion was found to be a prerequisite for E-cadherin transactivation in this model. In summary, the present study provides evidence that E-cadherin is an osmo-sensitive gene that responds to hypotonic stress. The function of this regulation may be found in morphological changes induced by cell swelling. It is likely that induction of E-cadherin contributes to the stabilization between adjacent cells in order to withstand the physical forces induced by hypotonicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 1, 3 January 2005, Pages 207-214
Journal: FEBS Letters - Volume 579, Issue 1, 3 January 2005, Pages 207-214
نویسندگان
Stefan Kippenberger, Stefan Loitsch, Maike Guschel, Jutta Müller, Roland Kaufmann, August Bernd,