کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10873609 1074283 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vivo involvement of mutated initiation factor IF1 in gene expression control at the translational level
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
In vivo involvement of mutated initiation factor IF1 in gene expression control at the translational level
چکیده انگلیسی
The influence in vivo of mutated forms of translation initiation factor (IF1) on the expression of the lacZ or 3A′ reporter genes, with different initiation and/or +2 codons, has been investigated. Reporter gene expression in these infA(IF1) mutants is similar to the wild-type strain. The results do not support the longstanding hypothesis that IF1 could perform discriminatory functions while blocking the aminoacyl-tRNA acceptor site (A-site) of the ribosome. One cold-sensitive IF1 mutant shows a general overexpression, in particular at low temperatures, of both reporter genes at the protein but not mRNA level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 579, Issue 5, 14 February 2005, Pages 995-1000
نویسندگان
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