Spatial distribution and ecological variation of re-discovered German truffle habitats

► We conducted a survey on truffle distribution and ecology in Southwest Germany. ► Seven species, all considered rare or even extinct, were discovered on 121 sites. ► The valuable Tuber aestivum was most abundant along wide ecological gradients. ► Climate change has probable effects on truffle ecology and distribution. ► Our results suggest ample truffle cultivation potential in the area.