کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10877052 | 1075904 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation of trehalose-6-phosphate phosphatase gene from tobacco and its functional analysis in yeast cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
Trehalose is a nonreducing disaccharide composed of two glucose units joined by an α, αâ1, 1 linkage, and has been found in bacteria, yeast, fungi, invertebrates and plants. Accumulation of trehalose in organisms plays a role in enhancing the stress tolerance. A trehalose-6-phosphate phosphatase gene, NtTPPL, was isolated from tobacco in this report. The predicted NtTPPL protein has a putative trehalose_PPase domain. The transcription of the NtTPPL gene was significantly induced by heat stress, and was only slightly induced by NaCl, PEG and low-temperature treatments. When expressing in yeast tps2 mutant, NtTPPL rescued the mutant phenotype under high temperature. This result indicated that NtTPPL functioned as a trehalose-6-phosphate phosphatase in yeast and may play similar roles in plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Plant Physiology - Volume 162, Issue 2, 21 February 2005, Pages 215-223
Journal: Journal of Plant Physiology - Volume 162, Issue 2, 21 February 2005, Pages 215-223
نویسندگان
Yu-Jun Wang, Yu-Jun Hao, Zhi-Gang Zhang, Tao Chen, Jin-Song Zhang, Shou-Yi Chen,