کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10878977 | 1076519 | 2014 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
First Evidence of Mariner-like Transposons in the Genome of the Marine Microalga Amphora acutiuscula (Bacillariophyta)
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: First Evidence of Mariner-like Transposons in the Genome of the Marine Microalga Amphora acutiuscula (Bacillariophyta) First Evidence of Mariner-like Transposons in the Genome of the Marine Microalga Amphora acutiuscula (Bacillariophyta)](/preview/png/10878977.png)
چکیده انگلیسی
Mariner-like elements (MLEs) are transposable elements able to move in the host genomes by a “cut and paste” mechanism. They have been found in numerous organisms. We succeeded in amplifying complete and truncated MLEs in the marine diatom Amphora acutiuscula. Full-length MLEs of 2,100Â bp delimited by imperfect Terminal Inverted Repeats revealed an intact Open Reading Frame, suggesting that the MLEs could be active. The DNA binding domain of the corresponding putative transposase could have two Helix-Turn-Helix and a Nuclear Location Site motifs, and its catalytic domain includes a particular triad of aspartic acids DD43D not previously reported. The number of copies was estimated to be 38, including approximately 20 full-length elements. Phylogenetic analysis shows that these peculiar MLEs differ from plant and other stramenopile MLEs and that they could constitute a new sub-family of Tc1-mariner elements.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protist - Volume 165, Issue 5, September 2014, Pages 730-744
Journal: Protist - Volume 165, Issue 5, September 2014, Pages 730-744
نویسندگان
Duc Hung Nguyen, Dorothée Hermann, Aurore Caruso, Emmanuelle Tastard, Justine Marchand, Jacques-Déric Rouault, Françoise Denis, Stanislas Thiriet-Rupert, Nathalie Casse, Annick Morant-Manceau,