Development of a monoclonal antibody against the left wing of ciguatoxin CTX1B: Thiol strategy and detection using a sandwich ELISA

► Thiol strategy for synthesizing a KLH-conjugate of the ABCDE-ring fragment of CTX1B. ► Producing a monoclonal antibody (3G8) against the left wing of CTX1B. ► Sandwich ELISA utilizing 3G8 and 8H4 enabled us to detect CTX1B specifically at less than 0.28 ng/mL.