Brown spider (Loxosceles intermedia) venom triggers endothelial cells death by anoikis

► We examined the mechanism of endothelial cell cytotoxicity induced by Loxosceles intermedia. ► Brown spider venom leads to important changes in cellular adhesion. ► The loss of cell anchorage provides the signal to initiate apoptosis. ► Venom triggers endothelial cell death by an apoptotic mechanism known as anoikis.