کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10880206 | 1076940 | 2012 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A nanoparticle-based bio-barcode assay for ultrasensitive detection of ricin toxin
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کلمات کلیدی
MmpsBCAGNPSReal-time PCR - PCR به موقعBio-barcode assay - آزمایش بیو بارکدELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاHigh-sensitivity detection - تشخیص حساسیت بالاRicin - ریسینMagnetic microparticles - میکرو ذرات مغناطیسیGold nanoparticles - نانوذرات طلاpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A nanoparticle-based bio-barcode assay for ultrasensitive detection of ricin toxin A nanoparticle-based bio-barcode assay for ultrasensitive detection of ricin toxin](/preview/png/10880206.png)
چکیده انگلیسی
The ultrasensitive bio-barcode amplification assay (BCA) technique was developed for the specific detection of the A chain of ricin toxin. The target antigen A chain was first captured by gold nanoparticles (GNPs) coated with polyclonal antibodies. Magnetic microparticles (MMPs) coated with A chain monoclonal antibody were then added to form a sandwich immuno-complex. After the immuno-complex was formed, signal DNA annealed to DNA strands covalently bound to the GNPs were released by heating and characterized by PCR and real-time fluorescence PCR. A detection limit of 1Â fg/ml was measured for A chain, six orders of magnitude more sensitive than that of conventional antigen-capture ELISA. The coefficient of variation (CV) of intra-assay and inter-assay ranged from 3.39% to 6.84%. The BCA can detect the A chain in milk and water mimic samples. In the following work it is demonstrated that this assay is a highly sensitive method for the detection of ricin proteins that could be adapted to measure other proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 59, Issue 1, January 2012, Pages 12-16
Journal: Toxicon - Volume 59, Issue 1, January 2012, Pages 12-16
نویسندگان
Hui-qiong Yin, Min-xian Jia, Shu Yang, Sheng-qi Wang, Jin-gang Zhang,