کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10881616 | 1077303 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Renal and antibacterial effects induced by myotoxin I and II isolated from Bothrops jararacussu venom
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Bothrops jararacussu myotoxin I (BthTx-I; Lys 49) and II (BthTX-II; Asp 49) were purified by ion-exchange chromatography and reverse phase HPLC. In this work we used the isolated perfused rat kidney method to evaluate the renal effects of B. jararacussu myotoxins I (Lys49 PLA2) and II (Asp49 PLA2) and their possible blockage by indomethacin. BthTX-I (5 μg/ml) and BthTX-II (5 μg/ml) increased perfusion pressure (PP; ct120=110.28±3.70 mmHg; BthTX I=171.28±6.30* mmHg; BthTX II=175.50±7.20* mmHg), renal vascular resistance (RVR; ct120=5.49 ±0.54 mmHg/ml.gâ1 minâ1; BthTX I=8.62±0.37* mmHg/ml gâ1 minâ1; BthTX II=8.9±0.36* mmHg/ml gâ1 minâ1), urinary flow (UF; ct120=0.14±0.01 ml gâ1 minâ1; BthTX I=0.32±0.05* ml gâ1 minâ1; BthTX II=0.37±0.01* ml gâ1 minâ1) and glomerular filtration rate (GFR; ct120=0.72±0.10 ml gâ1 minâ1; BthTX I=0.85±0.13* ml gâ1 minâ1; BthTX II=1.22±0.28* ml gâ1 minâ1). In contrast decreased the percent of sodium tubular transport (%TNa+; ct120=79,76±0.56; BthTX I=62.23±4.12*; BthTX II=70.96±2.93*) and percent of potassium tubular transport (%TK+;ct120=66.80±3.69; BthTX I=55.76±5.57*; BthTX II=50.86±6.16*). Indomethacin antagonized the vascular, glomerular and tubular effects promoted by BthTX I and it's partially blocked the effects of BthTX II. In this work also evaluated the antibacterial effects of BthTx-I and BthTx-II against Xanthomonas axonopodis. pv. passiflorae (Gram-negative bacteria) and we observed that both PLA2 showed antibacterial activity. Also we observed that proteins Also we observed that proteins chemically modified with 4-bromophenacyl bromide (Ï-BPB) decrease significantly the antibacterial effect of both PLA2. In conclusion, BthTx I and BthTX II caused renal alteration and presented activity antimicrobial. The indomethacin was able to antagonize totally the renal effects induced by BthTx I and partially the effects promoted by BthTx II, suggesting involvement of inflammatory mediators in the renal effects caused by myotoxins. In the other hand, other effects could be independently of the enzymatic activity of the BthTX II and the C-terminal domain could be involved in both effects promoted for PLA2.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 46, Issue 4, 15 September 2005, Pages 376-386
Journal: Toxicon - Volume 46, Issue 4, 15 September 2005, Pages 376-386
نویسندگان
P.S.F. Barbosa, A.M.C. Martins, A Havt, Daniela O. Toyama, J.S.A.M. Evangelista, D.P.P. Ferreira, P.P. Joazeiro, Luis O.S. Beriam, Marcos H. Toyama, M.C. Fonteles, H.S.A. Monteiro,