کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10889336 1080826 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improved antibody aggregate removal by hydroxyapatite chromatography in the presence of polyethylene glycol
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Improved antibody aggregate removal by hydroxyapatite chromatography in the presence of polyethylene glycol
چکیده انگلیسی
This paper reports a method for improving the effectiveness of aggregate removal from monoclonal antibodies by the use of hydroxyapatite (HA) in the presence of polyethylene glycol (PEG). PEG preferentially enhances aggregate retention, thereby increasing the degree of separation between aggregated and non-aggregated antibodies. PEG has a similar effect on ion exchangers but only half the enhancement observed with HA. The HA method is suitable for preparation of aggregate-free IgG and IgM monoclonal antibodies for research, diagnostic, or therapeutic applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 336, Issue 2, 31 July 2008, Pages 222-228
نویسندگان
,