کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10889345 | 1080828 | 2008 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid loss of dendritic cell and monocyte responses to TLR ligands following venipuncture
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rapid loss of dendritic cell and monocyte responses to TLR ligands following venipuncture Rapid loss of dendritic cell and monocyte responses to TLR ligands following venipuncture](/preview/png/10889345.png)
چکیده انگلیسی
Blood samples from multiple sites are collected in multicenter trials, and frequently shipped to centralized laboratories for processing and comparable experimental evaluation. It is therefore of crucial interest to assess the preservation of immune cell functions after overnight shipment of whole blood. Here we evaluated the ability of pDCs, mDCs and monocytes to respond to TLR ligands at multiple timepoints following venipuncture as compared to immediate processing. Our results demonstrate a profound impairment of APC function, in particular of IFN-α production of pDCs, if whole blood was processed later than 6 h after venipuncture. Overnight shipment or extended rest of whole blood before processing therefore severely compromises the ability of APCs to respond to TLR ligands, and this has to be taken into consideration when designing multicenter trials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 339, Issue 2, 31 December 2008, Pages 132-140
Journal: Journal of Immunological Methods - Volume 339, Issue 2, 31 December 2008, Pages 132-140
نویسندگان
Angela Meier, Amelia Fisher, Harlyn K. Sidhu, Judy J. Chang, Tom F. Wen, Hendrik Streeck, Galit Alter, Guido Silvestri, Marcus Altfeld,