Aging alters tissue resident mesenchymal stem cell properties

► Aging increases cell senescence factors like p16ink4a and CHEK1 and affect self-renewal of ASCs. ► These are followed by higher expression of DNA repair genes, suggestive of DNA break probabilities. ► Subnormal expression of microRNAs involved in cell cycle regulation was observed. ► Importantly, differentiation potential of ASCs declined with aging.