کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10891566 | 1082050 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of antifreeze glycoprotein 8 supplementation during vitrification on the developmental competence of bovine oocytes
ترجمه فارسی عنوان
تأثیر مکمل گلیکوپروتئین 8 ضد انجماد در طی شیشه سازی بر مهارت های رشد جوجه های گوشتی
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کلمات کلیدی
گلیکوپروتئین ضد انجماد 8، اووسیت، تبخیر کردن، یکپارچگی سی تی اسکلت، بارور شدن، رشد جنین،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
The purpose of this study was to investigate the effect of antifreeze glycoprotein 8 (AFGP8) supplementation during vitrification on the survival, fertilization, and embryonic development of bovine oocytes and the underlying molecular mechanism(s). Survival, fertilization, early embryonic development, apoptosis, DNA double-strand breaks, reactive oxygen species levels, meiotic cytoskeleton assembly, chromosome alignment, and energy status of mitochondria were measured in the present experiments. Compared with that in the nonsupplemented group; survival, monospermy, blastocyst formation rates, and blastomere counts were significantly higher in the AFGP8-supplemented animals. Oocytes of the latter group also presented fewer double-strand breaks and lower cathepsin B and caspase activities. Rates of normal spindle organization and chromosome alignment, actin filament impairment, and mitochondrial distribution were significantly higher in the AFGP8-supplemented group. In addition, intracellular reactive oxygen species levels significantly decreased in the AFGP8-supplemented groups, maintaining a higher ÎΨm than that in the nonsupplemented group. Taken together, these results indicated that supplementation with AFGP8 during vitrification has a protective effect on bovine oocytes against chilling injury.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 86, Issue 2, 15 July 2016, Pages 485-494.e1
Journal: Theriogenology - Volume 86, Issue 2, 15 July 2016, Pages 485-494.e1
نویسندگان
Shuang Liang, Bao Yuan, Jeong-Woo Kwon, Mija Ahn, Xiang-Shun Cui, Jeong Kyu Bang, Nam-Hyung Kim,