کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10891693 | 1082059 | 2015 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Motility and fertility of rabbit sperm cryopreserved using soybean lecithin as an alternative to egg yolk
ترجمه فارسی عنوان
تحرک و باروری اسپرم خرگوش با استفاده از لسیتین سویا به عنوان جایگزین برای زرده تخم مرغ
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
This study was conducted to investigate whether soy lecithin can be used as an alternative cryoprotectant to establish a procedure that does not require the use of egg yolk to cryopreserve rabbit strains. Semen from Japanese White rabbits was frozen with HEPES extender containing 20% egg yolk (EYH), 0.5% (Lec-0.5), 1.5% (Lec-1.5), 2.5% (Lec-2.5), or 3.5% (Lec-3.5; wt/vol) lecithin (type IV-S, â¥30%), and the motility of thawed sperm was analyzed. The sperm motility in the Lec-1.5 group was significantly higher than that in the Lec-2.5 and 3.5 groups and equivalent to the EYH group. From 17 rounds of artificial insemination with frozen-thawed sperm in the EYH and Lec-1.5 groups, 12 rabbits in both groups were pregnant (70.6%) and delivered offspring. The litter size was 3.3 in the EYH group and 5.1 in the Lec-1.5 group. These results indicate that soy lecithin can be used as a substitute for egg yolk as a cryoprotectant on the basis of motility and fertility of the frozen-thawed rabbit sperm and that 1.5% lecithin (type IV-S, â¥30%) in the semen extender was the optimum concentration for rabbit sperm cryopreservation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 84, Issue 7, 15 October 2015, Pages 1172-1175
Journal: Theriogenology - Volume 84, Issue 7, 15 October 2015, Pages 1172-1175
نویسندگان
Kazutoshi Nishijima, Shuji Kitajima, Chihiro Koshimoto, Masatoshi Morimoto, Teruo Watanabe, Jianglin Fan, Yukihisa Matsuda,