کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10892420 | 1082103 | 2012 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential expression of cyclic-AMP responsive element modulator (CREM) mRNA isoforms during testicular development of boars
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
The cyclic-AMP responsive element modulator (CREM) transcription factor, a key regulator of spermatogenesis, can activate or repress gene expression by differential expression of alternatively spliced mRNA isoforms. The objective of this study was to report the sequences of the predominant CREM mRNA isoforms with leader exons B, θ1, θ2, in addition to the relative expression of these transcripts, in immature and adult boar testes. Activator CREM isoforms Ïγ (containing the B exon), θ1Ïγ and θ2Ïγ, were expressed in the adult boar testes, but not in immature testes. The CREM θ2Ïγ isoform had the highest expression levels in the adult boar testes compared to the Ïγ and θ1Ïγ isoforms (θ2Ïγ = 4.94 ± 1.49 in contrast to Ïγ = 1.66 ± 0.79 and θ1Ïγ = 2.32 ± 0.72; mean ± SD; P < 0.01). Interestingly, the predominant CREM mRNA isoforms in adult boar testes contained the C and γ exons; these exons are not present in human and rodent CREM mRNA, demonstrating species-specific expression. In contrast to the adult CREM isoforms, only CREM mRNA isoforms with the B exon (Ï1 and α) were amplified in the 21 d (immature) boar testes. Further understanding of CREM mRNA isoform expression and regulation during boar spermatogenesis could contribute to our knowledge of spermatogenic efficiency in this species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 77, Issue 5, 15 March 2012, Pages 881-887
Journal: Theriogenology - Volume 77, Issue 5, 15 March 2012, Pages 881-887
نویسندگان
B.L. Sartini, M. Eerhart, S. Green,